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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA1-19168 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Vimentin Monoclonal Antibody (VI-01)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- VI-01
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Bioengineered uterine tissue supports pregnancy in a rat model.
Hellström M, Moreno-Moya JM, Bandstein S, Bom E, Akouri RR, Miyazaki K, Maruyama T, Brännström M
Fertility and sterility 2016 Aug;106(2):487-496.e1
Fertility and sterility 2016 Aug;106(2):487-496.e1
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human vimentin using mouse Monoclonal antibody VI-01 (Product # MA1-19168) on lysates of HeLa cell line and Raji cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-vimentin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for vimentin at approximately 55kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and western blot detection by VI-01 Monoclonal antibody (Product # MA1-19168). IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. MW of vimentin is 57 kDa. Lr = lysate (reducing conditions); Lnr = lysate (non-reducing conditions); IPr = immunoprecipitate (reducing conditions); IPnr = immunoprecipitate (non-reducing conditions).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human vimentin using mouse Monoclonal antibody VI-01 (Product # MA1-19168) on lysates of HeLa cell line and Raji cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-vimentin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for vimentin at approximately 55kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4). Lane 1,2: immunostaining with anti-Vimentin (VI-01) Monoclonal antibody (Product # MA1-19168); Lane 3,4: immunostaining with anti-human Vimentin (VI-RE/1).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1), Hep G2 (Lane 2), SHSY-5Y (Lane 3), A-431 (Lane 4) and HeLa (Lane 5). The blot was probed with Anti-Vimentin Monoclonal Antibody (Product # MA1-19168, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 54 kDa band corresponding to Vimentin was observed across all the cell lines positive for Vimentin (Lanes 1, 3 and 5) while this band was absent in the cell lines which do not express Vimentin protein (Lanes 2 and 4).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Vimentin using a monoclonal antibody (Product # MA1-19168).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Vimentin using a monoclonal antibody (Product # MA1-19168).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of vimentin in methanol/acetone fixed murine 3T3 cells using mouseMonoclonal antibody VI-01 (Product # MA1-19168).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- mmunofluorescence analysis of Vimentin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Vimentin Mouse Monoclonal Antibody (Product # MA1-19168) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L)/IgM (L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic, cytoskeletal and nuclear localization. Panel e represents negative control, A-431 cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining (paraffin sections) of vimentin in human liver using mouse Monoclonal antibody VI-01 (Product # MA1-19168) using a dilution of 1:400, detected with GAM IgM-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1 µg/mL; orange).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Vimentin using a monoclonal antibody (Product # MA1-19168).
