Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-14435 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Vimentin Antibody Cocktail
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-14435 targets Vimentin in IHC (P) applications and shows reactivity with Human samples.
- Antibody clone number
- Vimentin-SP20, gp100-HMB45
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts of HeLa (Lane 1), MDA-MB-231 (Lane 2), Daudi (Lane 3), Raji (Lane 4) and Ramos (Lane 5). The blot was probed with Anti-Vimentin Antibody Cocktail (Product # MA5-14435, 100 ul) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution), Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution) and Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 0.25 µg/ml, 1:4000 dilution) as the host species was mixed category. A 55 kDa band corresponding to Vimentin was observed across the cell lines tested except in the cell lines of the B-lymphocyte class.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed paraffin-embedded human melanoma stained with the MultiVision anti-Mouse/ HRP + anti-Rabbit/AP. Note: Cytoplasmic staining of Vimentin (in blue) and gp100 (in red).