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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- IC2105G - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Vimentin Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Vimentin in Western blots. Unconjugated antibody detects mouse and rat Vimentin in immunocytochemistry.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Green dye
- Antigen sequence
P08670- Isotype
- IgG
- Antibody clone number
- 280618
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references microRNA expression profile in Smooth Muscle Cells isolated from thoracic aortic aneurysm samples.
Kasprzyk-Pawelec A, Wojciechowska A, Kuc M, Zielinski J, Parulski A, Kusmierczyk M, Lutynska A, Kozar-Kaminska K
Advances in medical sciences 2019 Apr 22;64(2):331-337
Advances in medical sciences 2019 Apr 22;64(2):331-337
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Vimentin in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Rat Anti-Human/Mouse/Rat Vimentin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC2105G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
