GTX22737

antibody from GeneTex

Targeting: LRRC7 densin-180, KIAA1365

Western blot

Immunoprecipitation

Antibody data

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Validation data

Product number: GTX22737 - Provider product page
Provider: GeneTex
Proper citation: GeneTex Cat#GTX22737, RRID:AB_378982
Product name: LAP1 antibody [RL13]
Antibody type: Monoclonal
Reactivity: Human, Mouse, Rat
Host: Mouse
Storage: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

LRRC7 protein structure - GTX22737 shown in red.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemistry was performed on rat breast tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody (GTX22737) at a dilution of 1:50 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjμgated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemistry was performed on rat colon tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody (GTX22737) at a dilution of 1:20 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjμgated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemistry was performed on rat lymph node tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody (GTX22737) at a dilution of 1:50 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjμgated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.