Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-22259 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal DAP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DAP1 Antibody [NBP2-22259] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. NBP2-22259 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DAP1 Antibody [NBP2-22259] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: NBP2-22259 used for IP at 6 ug/mg lysate. DAP1 was also immunoprecipitated by another rabbit anti-DAP1 antibody. For blotting immunoprecipitated DAP1, NBP2-22259 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.