MA5-25245

antibody from Invitrogen Antibodies

Targeting: RBBP9 Bog

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA5-25245

Provider

Invitrogen Antibodies

Product name

RBBP9 Monoclonal Antibody (OTI4G9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI4G9

Vial size

100 µL

Concentration

0.67 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RBBP9 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with RBBP9 (Product # MA5-25245) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RBBP9 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with RBBP9 (Product # MA5-25245) monoclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RBBP9 in COS7 cells. Cells were transfected with a plasmid overexpressing RBBP9 and probed with a RBBP9 monoclonal antibody (Product # MA5-25245).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RBBP9 in COS7 cells. Cells were transfected with a plasmid overexpressing RBBP9 and probed with a RBBP9 monoclonal antibody (Product # MA5-25245).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RBBP9 in COS7 cells. Cells were transfected with a plasmid overexpressing RBBP9 and probed with a RBBP9 monoclonal antibody (Product # MA5-25245).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human bladder tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a RBBP9 monoclonal antibody (Product # MA5-25245) at a dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a RBBP9 monoclonal antibody (Product # MA5-25245) at a dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded carcinoma of human thyroid tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a RBBP9 monoclonal antibody (Product # MA5-25245) at a dilution of 1:50.