Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Other assay [2]
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- Product number
- 13-5800 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P-cadherin Monoclonal Antibody (NCC-CAD-299)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Source: Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of BALB/c mouse after immunization with human epidermal carcinoma cell A431.1) The monoclonal antibody was harvested from ascitic fluid. Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL).This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C. Specificity: This antibody specifically reacts with human P-cadherin. This antibody inhibits P-cadherin dependent cell-cell contact. Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- NCC-CAD-299
- Vial size
- 100 µg
- Concentration
- 2.0 mg/mL
- Storage
- 4° C
Submitted references An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice.
Extracellular cleavage and shedding of P-cadherin: a mechanism underlying the invasive behaviour of breast cancer cells.
Increased shedding of soluble fragments of P-cadherin in nipple aspirate fluids from women with breast cancer.
Cadherin switching dictates the biology of transitional cell carcinoma of the bladder: ex vivo and in vitro studies.
Smad4 induces the tumor suppressor E-cadherin and P-cadherin in colon carcinoma cells.
The fate of E- and P-cadherin during the early stages of apoptosis.
The small GTPases Rho and Rac are required for the establishment of cadherin-dependent cell-cell contacts.
The small GTPases Rho and Rac are required for the establishment of cadherin-dependent cell-cell contacts.
Hu JK, Du W, Shelton SJ, Oldham MC, DiPersio CM, Klein OD
Cell stem cell 2017 Jul 6;21(1):91-106.e6
Cell stem cell 2017 Jul 6;21(1):91-106.e6
Extracellular cleavage and shedding of P-cadherin: a mechanism underlying the invasive behaviour of breast cancer cells.
Ribeiro AS, Albergaria A, Sousa B, Correia AL, Bracke M, Seruca R, Schmitt FC, Paredes J
Oncogene 2010 Jan 21;29(3):392-402
Oncogene 2010 Jan 21;29(3):392-402
Increased shedding of soluble fragments of P-cadherin in nipple aspirate fluids from women with breast cancer.
Mannello F, Tonti GA, Medda V, Pederzoli A, Sauter ER
Cancer science 2008 Nov;99(11):2160-9
Cancer science 2008 Nov;99(11):2160-9
Cadherin switching dictates the biology of transitional cell carcinoma of the bladder: ex vivo and in vitro studies.
Bryan RT, Atherfold PA, Yeo Y, Jones LJ, Harrison RF, Wallace DM, Jankowski JA
The Journal of pathology 2008 Jun;215(2):184-94
The Journal of pathology 2008 Jun;215(2):184-94
Smad4 induces the tumor suppressor E-cadherin and P-cadherin in colon carcinoma cells.
Müller N, Reinacher-Schick A, Baldus S, van Hengel J, Berx G, Baar A, van Roy F, Schmiegel W, Schwarte-Waldhoff I
Oncogene 2002 Sep 5;21(39):6049-58
Oncogene 2002 Sep 5;21(39):6049-58
The fate of E- and P-cadherin during the early stages of apoptosis.
Schmeiser K, Grand RJ
Cell death and differentiation 1999 Apr;6(4):377-86
Cell death and differentiation 1999 Apr;6(4):377-86
The small GTPases Rho and Rac are required for the establishment of cadherin-dependent cell-cell contacts.
Braga VM, Machesky LM, Hall A, Hotchin NA
The Journal of cell biology 1997 Jun 16;137(6):1421-31
The Journal of cell biology 1997 Jun 16;137(6):1421-31
The small GTPases Rho and Rac are required for the establishment of cadherin-dependent cell-cell contacts.
Braga VM, Machesky LM, Hall A, Hotchin NA
The Journal of cell biology 1997 Jun 16;137(6):1421-31
The Journal of cell biology 1997 Jun 16;137(6):1421-31
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 6 Modulation of RT4 growth on collagen after 5 days. (A) RT4 growth without antibody (negative experimental control). (B) RT4 growth in the presence of anti-CK20 antibody (negative antibody control). (C) RT4 growth in the presence of anti-P-cadherin function-blocking antibody. (A-C) All show RT4 forming tight rounded multicellular colonies without any noticeable morphological changes. (D) RT4 growth in the presence of anti-E-cadherin function-blocking antibody, with the cells clustering together but seemingly unable to form tight rounded multicellular colonies
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 5 Modulation of RT4 growth on plastic after 24 h. (A) RT4 growth without antibody (negative experimental control). (B) RT4 growth in the presence of anti-CK20 antibody (negative antibody control). (C, D) RT4 growth in the presence of anti-P-cadherin function blocking antibody. (E, F) RT4 growth in the presence of anti-E-cadherin function-blocking antibody