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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
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- Product number
- MA1-2003 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P-cadherin Monoclonal Antibody (6A9)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-2003 detects P-cadherin from human and mouse samples. MA1-2003 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~120 kDa protein representing P-cadherin from A431 cell extract. MA1-2003 can also be used in immunoprecipitation and immunofluorescence procedures. The MA1-2003 immunogen is A431 trypsinized membranes.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6A9
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Connexins, E-cadherin, Claudin-7 and β-catenin transiently form junctional nexuses during the post-natal mammary gland development.
Mechanism of extracellular domain-deleted dominant negative cadherins.
Mechanism of extracellular domain-deleted dominant negative cadherins.
Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes.
Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes.
Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains.
Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.
Dianati E, Poiraud J, Weber-Ouellette A, Plante I
Developmental biology 2016 Aug 1;416(1):52-68
Developmental biology 2016 Aug 1;416(1):52-68
Mechanism of extracellular domain-deleted dominant negative cadherins.
Nieman MT, Kim JB, Johnson KR, Wheelock MJ
Journal of cell science 1999 May;112 ( Pt 10):1621-32
Journal of cell science 1999 May;112 ( Pt 10):1621-32
Mechanism of extracellular domain-deleted dominant negative cadherins.
Nieman MT, Kim JB, Johnson KR, Wheelock MJ
Journal of cell science 1999 May;112 ( Pt 10):1621-32
Journal of cell science 1999 May;112 ( Pt 10):1621-32
Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes.
Hines MD, Jin HC, Wheelock MJ, Jensen PJ
Journal of cell science 1999 Dec;112 ( Pt 24):4569-79
Journal of cell science 1999 Dec;112 ( Pt 24):4569-79
Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes.
Hines MD, Jin HC, Wheelock MJ, Jensen PJ
Journal of cell science 1999 Dec;112 ( Pt 24):4569-79
Journal of cell science 1999 Dec;112 ( Pt 24):4569-79
Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains.
Wahl JK, Sacco PA, McGranahan-Sadler TM, Sauppé LM, Wheelock MJ, Johnson KR
Journal of cell science 1996 May;109 ( Pt 5):1143-54
Journal of cell science 1996 May;109 ( Pt 5):1143-54
Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.
Islam S, Carey TE, Wolf GT, Wheelock MJ, Johnson KR
The Journal of cell biology 1996 Dec;135(6 Pt 1):1643-54
The Journal of cell biology 1996 Dec;135(6 Pt 1):1643-54
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cadherin P was performed by loading 25 µg of 293T (lane 1), A431 (lane 2) and mouse brain (lane 3) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Cadherin P monoclonal antibody (Product # MA1-2003) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~120 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of placental Cadherin using Cadherin P Monoclonal Antibody (6A9) (Product # MA1-2003) shows staining in A2058 Cells. Cadherin P (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Cadherin P (Product # MA1-2003) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of placental Cadherin using Cadherin P Monoclonal Antibody (6A9) (Product # MA1-2003) shows staining in BEAS-2B Cells. Cadherin P (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Cadherin P (Product # MA1-2003) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of placental Cadherin using Cadherin P Monoclonal Antibody (6A9) (Product # MA1-2003) shows staining in Hela Cells. Cadherin P (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Cadherin P (Product # MA1-2003) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.
