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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- AF861 - Provider product page
- Provider
- R&D Systems
- Product name
- Human P-Cadherin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human P-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Cadherin-11, rhCadherin-12, rhCadherin-13, rhE-Cadherin and less than 1% cross-reactivity with rhCadherin-8, rhCadherin-17, rhN-Cadherin, rhK-Cadherin and recombinant mouse P-Cadherin is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
CAA45177- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Trophoblast differentiation defect in human embryonic stem cells lacking PIG-A and GPI-anchored cell-surface proteins.
Chen G, Ye Z, Yu X, Zou J, Mali P, Brodsky RA, Cheng L
Cell stem cell 2008 Apr 10;2(4):345-55
Cell stem cell 2008 Apr 10;2(4):345-55
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human P-Cadherin by Simple WesternTM. Simple Western lane view shows lysates of ZR-75 human breast cancer cell line and A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for P-Cadherin at approximately 135 kDa (as indicated) using 10 µg/mL of Goat Anti-Human P-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF861) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human P-Cadherin by Western Blot. Western blot shows lysate of ZR-75 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human P-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF861) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for P-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
