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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-2450 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal NHERF-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Please note that this variant has an extra insertion of 11 residues compared to NP_004776.3. The immunizing peptide represents this insertion.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references cGMP inhibition of Na+/H+ antiporter 3 (NHE3) requires PDZ domain adapter NHERF2, a broad specificity protein kinase G-anchoring protein.
Cha B, Kim JH, Hut H, Hogema BM, Nadarja J, Zizak M, Cavet M, Lee-Kwon W, Lohmann SM, Smolenski A, Tse CM, Yun C, de Jonge HR, Donowitz M
The Journal of biological chemistry 2005 Apr 29;280(17):16642-50
The Journal of biological chemistry 2005 Apr 29;280(17):16642-50
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NHERF-2 Antibody [NB100-2450] - (0.3ug/ml) Analysis of human kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NHERF-2 Antibody [NB100-2450] - IHC analysis of NHERF-2 in paraffin embedded Human Kidney using NB100-2450 at 3ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
