PA5-78487

antibody from Invitrogen Antibodies

Targeting: SOX8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78487

Provider

Invitrogen Antibodies

Product name

SOX8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: U87-MG, SK-N-SH, IMR32, SK-N-AS, human brain

Concentration

1.59 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SOX8 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with SOX8 polyclonal antibody (Product # PA5-78487) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SOX8 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with SOX8 polyclonal antibody (Product # PA5-78487) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SOX8 Polyclonal Antibody detects SOX8 protein by western blot analysis. Human tissue extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX8 Polyclonal Antibody (Product # PA5-78487) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SOX8 Polyclonal Antibody detects SOX8 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX8 Polyclonal Antibody (Product # PA5-78487) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SOX8 Polyclonal Antibody detects SOX8 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SOX8 protein stained by SOX8 Polyclonal Antibody (Product # PA5-78487) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SOX8 Polyclonal Antibody detects SOX8 protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SOX8 Polyclonal Antibody (Product # PA5-78487) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.