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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- RQ7231 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- KLF13 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific KLF13 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the KLF13 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human SH-SY5Y, 2) human HeLa, 3) human HEL, 4) human HL60, 5) human PC-3, 6) human Jurkat, 7) rat thymus and 8) mouse thymus tissue lysate with KLF13 antibody. Predicted molecular weight ~31 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat thymus tissue with KLF13 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HeLa cells with KLF13 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KLF13 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse Neuro-2a cells with KLF13 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KLF13 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of rat C6 cells with KLF13 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KLF13 antibody.
