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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PB10016 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Mast Cell Tryptase/TPSAB1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for TPS1/TPSAB1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. TPS1/TPSAB1 information: Molecular Weight: 30515 MW; Subcellular Localization: Secreted. Released from the secretory granules upon mast cell activation; Tissue Specificity: Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells (at protein level).
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Mast Cell Tryptase expression in 293T whole cell lysates (lane 1). Mast Cell Tryptase at 30KD was detected using rabbit anti- Mast Cell Tryptase Antigen Affinity purified polyclonal antibody (Catalog # PB10016) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image
