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Learn13343-1-AP
antibody from Invitrogen Antibodies
Targeting: TPSAB1
TPS1, TPS2, TPSB1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 13343-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TPSAB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLSLLLLAL PVLASPAYAA PAPVQALQQA GIVGGQEAPR SKWPWQVSLR VRDRYWMHFC GGSLIHPQWV LTAAHCLGPD VKDLATLRVQ LREQHLYYQD QLLPVSRIIV HPQFYIIQTG ADIALLELEE PVNISSRVHT VMLPPASETF PPGMPCWVTG WGDVDNDEPL PPPFPLKQVK VPIMENHICD AKYHLGAYTG DDVRIIRDDM LCAGNTRRDS CQGDSGGPLV CKVNGTWLQA GVVSWDEGCA QPNRPGIYTR VTYYLDWIHH YVPKKP (1-275 aa encoded by B C028059)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse colon tissue were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 13343-1-AP (TPSAB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Citric acid buffer, pH 6.0.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Citric acid buffer, pH 6.0.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human APpendix using 13343-1-AP (TPSAB1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human APpendix using 13343-1-AP (TPSAB1 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 U-937 cells were intracellularly stained with 0.2 µg Anti-Human TPSAB1 (Product # 13343-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-TPSAB1 (IP:13343-1-AP, 4ug; Detection:13343-1-AP 1:800) with mouse liver tissue lysate 4000ug.
