MA5-11711

antibody from Invitrogen Antibodies

Targeting: TPSAB1 TPS1, TPS2, TPSB1

Western blot

Antibody data

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Validation data

Product number: MA5-11711 - Provider product page
Provider: Invitrogen Antibodies
Product name: Tryptase Monoclonal Antibody (AA1)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-11711 targets Mast Cell Tryptase in IHC (P) and WB applications and shows reactivity with Canine, Feline, Human, and Non-human primate samples. The MA5-11711 immunogen is human mast cell tryptase purified from human lung tissues.
Reactivity: Human, Canine, Feline
Host: Mouse
Isotype: IgG
Antibody clone number: AA1
Vial size: 500 µL
Concentration: 0.05 mg/mL
Storage: 4° C

TPSAB1 protein structure - MA5-11711 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 2 Characterization of mast cells derived from bone marrow CD34+ hematopoietic precursors. A. Immunofluorescence staining of original CD34+ hematopoietic precursors and differentiated mast cells. Anti-tryptase antibody or anti-chymase antibody was used in the experiments. DAPI was used to stain the nucleus of the cells. B. Quantification of experiments described in A, more than 200 cells were counted in each group, mean+-SD, n = 3. C. Toluidine blue staining of the derived mast cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 5 MIF knockdown inhibits the profibrogenic activity of Mast cells. A and B. The mRNA and protein levels of MIF in mast cells derived from CD34+ hematopoietic precursors with or without MIF knockdown. C. Western-blots of chymase, tryptase and GAPDH in original or mast cells (derived from CD34+ hematopoietic precursors with or without MIF knockdown). D. Toluidine blue staining of the mast cells derived from CD34+ hematopoietic precursors with or without MIF knockdown. E. Quantification analysis of FcepsilonRI/CD117 double positive population in original or mast cells (derived from CD34+ hematopoietic precursors with or without MIF knockdown), #P>0.05. F. Histamine release assay of the mast cells derived from CD34+ hematopoietic precursors with or without MIF knockdown. G and H. The growth curves and collagen synthesis of fibroblast cultured in medium conditioned by mast cells with or without MIF knockdown, mean+-SD, n = 3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Representative slides of IHC staining with anti-FCERI and anti-TPSAB1 to visualize mast cells in osteophyte sections. Representative areas of osteophytes of study patients are shown as P1, P2 and P3. Antibody-stained mast cells were localized in bone trabeculae, cartilage region, where the columnar chondrocytes can be seen. MC are mast cells at 100X magnification revealing granular features. Bottom panel: histograms--control and osteophytes, denote anti-FCERI and anti-TPSAB1 stained cells (%) respectively; * p < 0.05, ** p < 0.01 compared to control.