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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [8]
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- Product number
- LS-C379892 - Provider product page
- Provider
- LSBio
- Product name
- SIRT3 / Sirtuin 3 Antibody (aa20-130) LS-C379892
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot. All lanes: NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody at 1 ug/ml+HepG2 whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 44 kDa. Observed band size: 44 kDa Immunohistochemistry.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: SIRT3 antibody at 0.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 29 kDa Observed band size: 44 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using antibody at 1:100 dilution.
