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- Product number
- PA5-86035 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIRT3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references SIRT3 and Metabolic Reprogramming Mediate the Antiproliferative Effects of Whey in Human Colon Cancer Cells.
D'Onofrio N, Martino E, Balestrieri A, Mele L, Neglia G, Balestrieri ML, Campanile G
Cancers 2021 Oct 16;13(20)
Cancers 2021 Oct 16;13(20)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of SIRT3 in MCF-7 cells using a SIRT3 Polyclonal antibody (Product # PA5-86035)..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of SIRT3 in HepG2 cells using a SIRT3 Polyclonal antibody (Product # PA5-86035)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of SIRT3 in HepG2 cells using a SIRT3 Polyclonal Antibody (Product # PA5-86035) at a dilution of 1:50, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Antiproliferative effects of whey are mediated by SIRT3 modulation. SIRT3 protein, mRNA levels and enzyme activity were assessed on ( A - D ) HCT 116, ( E - H ) HT-29, ( I - L ) SW480 and ( M - P ) LoVo cells treated with whey (40% v / v ). Lane 1 = molecular markers; Lane 2 = 0 h; lane 3 = 48 h; lane 4 = 72 h. SIRT3 protein expression was normalized against gamma-tubulin and determined with ImageJ software and values expressed as arbitrary units (AU). For PCR products, SIRT3 mRNA levels were normalized against GAPDH internal control and the amplified transcripts are shown with the expected sizes on 2.0% agarose gels. * p < 0.05 vs. 0 h; ++ p < 0.01 vs. 0 h; SS p < 0.001 vs. 0 h.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8 Whey effects in HCT 116 and HT-29 cells occur via SIRT3. Cells were transfected with scramble siRNA (NT-siRNA), transfection reagent (Lipofectamin) and SIRT3 siRNA, as described under Methods section. Protein expression levels of SIRT3 in ( A , B ) HCT 116 and ( C , D ) HT-29. Lane 1 = molecular markers; Lane 2 = Ctr; lane 3 = NT-siRNA; lane 4 = Lipofectamin; Lane 5 = SIRT3 siRNA. SS p < 0.001 vs. Ctr. After SIRT3 silencing, HCT 116 and HT-29 cells were treated with whey (40% v / v ) for 72 h and ( E - H ) LDHA, ( I - L ) PPAR-gamma, and ( M , P ) PPAR-alpha expression levels were determined. Lane 1 = molecular markers; Lane 2 = Ctr; lane 3 = NT-siRNA; lane 4 = SIRT3 siRNA; lane 5 = whey 40% v / v for 72 h; lane 6 = SIRT3 siRNA + whey 40% v / v for 72 h. The protein levels were normalized using gamma-tubulin as internal control with Image J software and values expressed as arbitrary units (AU). SS p < 0.001 vs. Ctr; # p < 0.05 vs. NT-siRNA; ## p < 0.01 vs. NT-siRNA.
