PA5-117095

antibody from Invitrogen Antibodies

Targeting: SIRT3 SIR2L3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117095

Provider

Invitrogen Antibodies

Product name

SIRT3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: A-549, 22Rv1, SW620, MCF7 Immunogen sequence: QRETGKLDGP DK

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

3.25 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SIRT3 in extracts of various cell lines. Samples were incubated with SIRT3 Polyclonal antibody (Product # PA5-117095) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SIRT3 in NIH/3T3 cells. Samples were incubated with SIRT3 Polyclonal antibody (Product # PA5-117095) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of SIRT3 in 300 μg extracts of Hela cells. Samples were precipitated with 3 μg SIRT3 Polyclonal antibody (Product # PA5-117095). Western blot was performed from the immunoprecipitate using SIRT3 Polyclonal antibody (Product # PA5-117095) at a dilution of 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SIRT3 in paraffin-embedded mouse kidney. Samples were incubated with SIRT3 Polyclonal antibody (Product # PA5-117095) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SIRT3 in paraffin-embedded rat kidney. Samples were incubated with SIRT3 Polyclonal antibody (Product # PA5-117095) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SIRT3 in paraffin-embedded human colon carcinoma. Samples were incubated with SIRT3 Polyclonal antibody (Product # PA5-117095) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SIRT3 in paraffin-embedded human colon carcinoma. Samples were incubated with SIRT3 Polyclonal antibody (Product # PA5-117095) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of SIRT3 in 300 µg extracts of HeLa cells using SIRT3 Polyclonal Antibody (Product # PA5-117095). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:500