PA5-117095

antibody from Invitrogen Antibodies

Targeting: SIRT3 SIR2L3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117095

Provider

Invitrogen Antibodies

Product name

SIRT3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: A-549, 22Rv1, SW620, MCF7 Immunogen sequence: QRETGKLDGP DK

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

3.25 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SIRT3 using SIRT3 Polyclonal Antibody (Product # PA5-117095) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SIRT3 using SIRT3 Polyclonal Antibody (Product # PA5-117095) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of SIRT3 in human colon carcinoma tissue using SIRT3 Polyclonal Antibody (Product # PA5-117095) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of SIRT3 in rat heart tissue using SIRT3 Polyclonal Antibody (Product # PA5-117095) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of SIRT3 in mouse heart tissue using SIRT3 Polyclonal Antibody (Product # PA5-117095) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of SIRT3 in 300 µg extracts of HeLa cells using SIRT3 Polyclonal Antibody (Product # PA5-117095). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:500