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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ANC-007-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nicotinic Acetylcholine Receptor alpha 7 (CHRNA7) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates: - 1,2. Anti-Nicotinic Acetylcholine Receptor alpha 7 (CHRNA7) (extracellular) Antibody (#ANC-007), (1:200).3,4. Anti-Nicotinic Acetylcholine Receptor alpha 7 (CHRNA7) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 (extracellular) Blocking Peptide (#BLP-NC007).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of nAChR alpha 7 in rat PC12 cells - Cell surface detection of nAChR alpha 7 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Nicotinic Acetylcholine Receptor alpha 7 (CHRNA7) (extracellular) Antibody (#ANC-007), (1:25), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live view of the cells. C. Merge of A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of nAChR alpha 7 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Nicotinic Acetylcholine Receptor alpha 7 (CHRNA7) (extracellular) Antibody (#ANC-007), (1:200). nAChR alpha 7 (red staining) is expressed in DRG neurons. Hoechst 33342 is used as the counterstain (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection ofnAChR alpha 7by indirect flow cytometry in live intact human THP-1monocyticleukemia cells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells + Anti-Nicotinic Acetylcholine Receptor alpha 7 (CHRNA7) (extracellular) Antibody (#ANC-007), (2.5μg) + goat- Anti-rabbit-FITC.
