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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MA5-42563 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAM62B Monoclonal Antibody (C4-D0)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Siha cell lysate, THP-1 cell lysate, human colon carcinoma tissue. Subcellular Location: Cell membrane, Endoplasmic reticulum, Membrane.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- C4-D0
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded human colon carcinoma tissue using FAM62B Monoclonal Antibody (Product # MA5-42563). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the FAM62B antibody at a dilution of 1:100 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
