HPA002132

antibody from Atlas Antibodies

Targeting: ESYT2 CHR2SYT, FAM62B, KIAA1228

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA002132

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA002132, RRID:AB_1078807

Product name

Anti-ESYT2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

FTLDEVPKGKLHLRLEWLTLMPNASNLDKVLTDIK
ADKDQANDGLSSALLILYLDSARNLPSGKKISSNP
NPVVQMSVGHKAQESKIRYKTNEPVWEENFTFFIH
NPKRQDLEVEVRDEQHQ


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Three-dimensional architecture of extended synaptotagmin-mediated endoplasmic reticulum–plasma membrane contact sites

Fernández-Busnadiego R, Saheki Y, De Camilli P
Proceedings of the National Academy of Sciences 2015 April;112(16)

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ESYT2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line SK-BR-3.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows weak cytoplasmic/membranous positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows moderate cytoplasmic/membranous positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.

Sample type

HUMAN