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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-87880 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZIP14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: LO2, HeLa; Cellular Location: Cell membrane, Cell projection, Multi-pass membrane protein, lamellipodium Immunogen sequence: EENEQTEEGR PSAVE
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.6 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Human placental cell line HTR-8/SVneo accumulates cadmium by divalent metal transporters DMT1 and ZIP14.
Widhalm R, Ellinger I, Granitzer S, Forsthuber M, Bajtela R, Gelles K, Hartig PY, Hengstschläger M, Zeisler H, Salzer H, Gundacker C
Metallomics : integrated biometal science 2020 Nov 1;12(11):1822-1833
Metallomics : integrated biometal science 2020 Nov 1;12(11):1822-1833
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using SLC39A14 Polyclonal antibody (Product # PA5-87880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZIP14 in extracts of various cell lines using ZIP14 Polyclonal Antibody (Product # PA5-87880) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ZIP14 in paraffin-embedded human liver cancer. Samples were incubated with ZIP14 Polyclonal antibody (Product # PA5-87880) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ZIP14 in paraffin-embedded rat liver. Samples were incubated with ZIP14 Polyclonal antibody (Product # PA5-87880) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
