PA5-21077

antibody from Invitrogen Antibodies

Targeting: SLC39A14 KIAA0062, NET34, ZIP14

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21077

Provider

Invitrogen Antibodies

Product name

ZIP14 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Suggested positive controls are human liver, mouse kidney, or rat kidney tissue lysates. PA5-21077 can be used with blocking peptide PEP-1191. Predicted species reactivity based on immunogen sequence: Bovine: (94%). At least three isoforms of ZIP14 are known to exist; this antibody will detect all isoforms. ZIP14 antibody is predicted to not cross-react with other ZIP family members.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa.

Protic I, Golic I, Vidakovic S, Korac B, Korac A
Current issues in molecular biology 2022 Jul 31;44(8):3444-3454

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Transferrin receptor 1 ablation in satellite cells impedes skeletal muscle regeneration through activation of ferroptosis.

Ding H, Chen S, Pan X, Dai X, Pan G, Li Z, Mai X, Tian Y, Zhang S, Liu B, Cao G, Yao Z, Yao X, Gao L, Yang L, Chen X, Sun J, Chen H, Han M, Yin Y, Xu G, Li H, Wu W, Chen Z, Lin J, Xiang L, Hu J, Lu Y, Zhu X, Xie L
Journal of cachexia, sarcopenia and muscle 2021 Jun;12(3):746-768

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Prolonged stimulation of insulin release from MIN6 cells causes zinc depletion and loss of β-cell markers.

Lawson R, Maret W, Hogstrand C
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) 2018 Sep;49:51-59

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Increased free Zn(2+) correlates induction of sarco(endo)plasmic reticulum stress via altered expression levels of Zn(2+) -transporters in heart failure.

Olgar Y, Durak A, Tuncay E, Bitirim CV, Ozcinar E, Inan MB, Tokcaer-Keskin Z, Akcali KC, Akar AR, Turan B
Journal of cellular and molecular medicine 2018 Mar;22(3):1944-1956

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Conditional mouse models support the role of SLC39A14 (ZIP14) in Hyperostosis Cranialis Interna and in bone homeostasis.

Hendrickx G, Borra VM, Steenackers E, Yorgan TA, Hermans C, Boudin E, Waterval JJ, Jansen IDC, Aydemir TB, Kamerling N, Behets GJ, Plumeyer C, D'Haese PC, Busse B, Everts V, Lammens M, Mortier G, Cousins RJ, Schinke T, Stokroos RJ, Manni JJ, Van Hul W
PLoS genetics 2018 Apr;14(4):e1007321

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Iron importers Zip8 and Zip14 are expressed in retina and regulated by retinal iron levels.

Sterling J, Guttha S, Song Y, Song D, Hadziahmetovic M, Dunaief JL
Experimental eye research 2017 Feb;155:15-23

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of mouse liver cells using a ZIP14 polyclonal antibody (Product # PA5-21077) at a 20 µg/mL dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence detection of ZIP14 transporter in normozoospermic ( a ) and asthenozoospermic ( c ) sperm samples using a confocal laser scanning microscope. Nc--negative control. Scale bar = 10 um. Average of total number of spermatozoa per ejaculate before processing was in normo- (625 x 10 6 /vol) and asthenozoospermia (367.5 x 10 6 /vol); For each type of sample, six randomly selected field areas were analysed (>100 cells). Relative immunopositivity of ZIP14 among the compartments in the normozoospermic ( b ) and asthenozoospermic ( d ) sperm samples. * in comparison to head, *** p

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Expression levels of ZIP 8, ZIP 14 and ZnT8 in heart tissue homogenates and doxorubicin-treated H9c2 cells. Representative Western blotting bands for protein expression levels are given left part of figures in ( A ) and ( B ). The densitometry analysis showing the proteins of ZIP 8, ZnT8 and ZIP 14 is expressed at 50 kD, 41 kD and 50 kD, respectively, in doxorubicin-treated H9c2 cells ( DOXO group) with respect to untreated cells ( CON group) ( A ) and underwent orthotopic heart transplantation with end-stage heart failure ( HF group) with respect to unsuitable for cardiac transplantation patients ( CON group) ( B ) GAPDH at 37 kD as reference protein. Bars represent mean (+-S.E.M.) for each group. Number of human samples, n = 3 for HF group and n = 2 for CON group. All measurements with double assays in each sample from each group for each type of measurement. Significance level accepted at * P < 0.05 versus CON.