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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- PA5-51699 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GOLIM4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ENRQLRKAHQ DIHTQLQDVK QQHKNLLSEH EQLVVTLEDH KSALAAAQTQ VAEYKQLKDT LNRIPSLRKP DPAEQQNVTQ VAHSPQGYNT AREKPTREVQ EVSRNNDVWQ Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 82%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of GOLIM4 in human cell line U-251 MG shows positivity in the Golgi apparatus & nucleus but excluded from the nucleoli. Samples were probed using a GOLIM4 Polyclonal Antibody (Product # PA5-51699).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GOLIM4 in human small intestine and skin tissues using GOLIM4 Polyclonal Antibody (Product # PA5-51699). Corresponding GOLIM4 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GOLIM4 in human cerebral cortex, liver, skin and small intestine using GOLIM4 Polyclonal Antibody (Product # PA5-51699) (A) shows similar protein distribution across tissues to an independent GOLIM4 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GOLIM4 in human skin using GOLIM4 Polyclonal Antibody (Product # PA5-51699) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GOLIM4 in human small intestine using GOLIM4 Polyclonal Antibody (Product # PA5-51699) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GOLIM4 in human cerebral cortex using GOLIM4 Polyclonal Antibody (Product # PA5-51699).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GOLIM4 in human liver using GOLIM4 Polyclonal Antibody (Product # PA5-51699).
