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Western blot
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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA016816 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA016816, RRID:AB_1858393
- Product name
- Anti-RSPH1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human RSPH1, Gene description: radial spoke head 1 homolog (Chlamydomonas), Alternative Gene Names: CILD24, FLJ32753, RSP44, RSPH10A, TSGA2, Validated applications: IHC, WB, Uniprot ID: Q8WYR4, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Rsph4a is essential for the triplet radial spoke head assembly of the mouse motile cilia
Immunofluorescence Analysis and Diagnosis of Primary Ciliary Dyskinesia with Radial Spoke Defects.
Antibody-based Protein Profiling of the Human Chromosome 21
Omran H, Yoke H, Ueno H, Narita A, Sakai T, Horiuchi K, Shingyoji C, Hamada H, Shinohara K
PLOS Genetics 2020;16(3):e1008664
PLOS Genetics 2020;16(3):e1008664
Immunofluorescence Analysis and Diagnosis of Primary Ciliary Dyskinesia with Radial Spoke Defects.
Frommer A, Hjeij R, Loges NT, Edelbusch C, Jahnke C, Raidt J, Werner C, Wallmeier J, Große-Onnebrink J, Olbrich H, Cindrić S, Jaspers M, Boon M, Memari Y, Durbin R, Kolb-Kokocinski A, Sauer S, Marthin JK, Nielsen KG, Amirav I, Elias N, Kerem E, Shoseyov D, Haeffner K, Omran H
American journal of respiratory cell and molecular biology 2015 Oct;53(4):563-73
American journal of respiratory cell and molecular biology 2015 Oct;53(4):563-73
Antibody-based Protein Profiling of the Human Chromosome 21
Uhlén M, Oksvold P, Älgenäs C, Hamsten C, Fagerberg L, Klevebring D, Lundberg E, Odeberg J, Pontén F, Kondo T, Sivertsson Å
Molecular & Cellular Proteomics 2012;11(3):M111.013458
Molecular & Cellular Proteomics 2012;11(3):M111.013458
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and RSPH1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409012).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human fallopian tube and cerebral cortex tissues using HPA016816 antibody. Corresponding RSPH1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
