HPA017382

RSPH1 antibody from Atlas Antibodies

CILD24, FLJ32753, RSP44, RSPH10A, TSGA2

Eligible for validation within the Antibodypedia Validation Initiative

Immunohistochemistry

Enhanced validation

Provider product page for HPA017382

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Antibody Data

Product number

HPA017382

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA017382, RRID:AB_1236007

Product name

Anti-RSPH1

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

TAELIHLNHRYQGKFLNKNPVGPGKYVFDVGCEQH
GEYRLTDMERGEEEEEEELVTVVPKWKATQITELA
LWTPTL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Targeted NGS gene panel identifies mutations in RSPH1 causing primary ciliary dyskinesia and a common mechanism for ciliary central pair agenesis due to radial spoke defects
Onoufriadis A, Shoemark A, Schmidts M, Patel M, Jimenez G, Liu H, Thomas B, Dixon M, Hirst R, Rutman A, Burgoyne T, Williams C, Scully J, Bolard F, Lafitte J, Beales P, Hogg C, Yang P, Chung E, Emes R, O'Callaghan C, Bouvagnet P, Mitchison H
Human Molecular Genetics 2014 June;23(13):3362-3374

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Loss-of-Function Mutations in RSPH1 Cause Primary Ciliary Dyskinesia with Central-Complex and Radial-Spoke Defects
Kott E, Legendre M, Copin B, Papon J, Dastot-Le Moal F, Montantin G, Duquesnoy P, Piterboth W, Amram D, Bassinet L, Beucher J, Beydon N, Deneuville E, Houdouin V, Journel H, Just J, Nathan N, Tamalet A, Collot N, Jeanson L, Le Gouez M, Vallette B, Vojtek A, Epaud R, Coste A, Clement A, Housset B, Louis B, Escudier E, Amselem S
The American Journal of Human Genetics 2013 September;93(3):561-570

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows cytoplasmic positivity in spermatocytes and spermatides.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex using Anti-RSPH1 antibody HPA017382.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-RSPH1 antibody HPA017382.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human fallopian tube and placenta tissues using Anti-RSPH1 antibody. Corresponding RSPH1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex, fallopian tube, placenta and testis using Anti-RSPH1 antibody HPA017382 (A) shows similar protein distribution across tissues to independent antibody HPA016816 (B).

Sample type

HUMAN

Antibody #2 product nr

HPA016816

Antibody provider

Atlas Antibodies

Independent Antibody Method

ANTIBODY

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