MA5-42520

antibody from Invitrogen Antibodies

Targeting: MBD2

Western blot

Antibody data

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Validation data

Product number: MA5-42520 - Provider product page
Provider: Invitrogen Antibodies
Product name: MBD2 Recombinant Rabbit Monoclonal Antibody (JE57-73)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: Positive Control: Raji cell lysates, rat kidney tissue, human lung carcinoma tissue, human breast carcinoma tissue, Daudi. Subcellular Location: Nucleus.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Antibody clone number: JE57-73
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MBD2 protein structure - MA5-42520 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat kidney tissue using MBD2 Recombinant Monoclonal Antibody (Product # MA5-42520). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the MBD2 antibody at a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of paraffin-embedded human lung carcinoma tissue using MBD2 Recombinant Monoclonal Antibody (Product # MA5-42520). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the MBD2 antibody at a dilution of 1:200 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of paraffin-embedded human breast carcinoma tissue using MBD2 Recombinant Monoclonal Antibody (Product # MA5-42520). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the MBD2 antibody at a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.