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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-89229 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHF11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: IAQSAKFSGV KRKRGRKKPL SGNHVQPPET MKCNTFIRQV KEEHGRHTDA TVKVPFLKKC KEAGLLNYLL EEILDKVHSI PEKLMDETTS ESDYEEIGSA LFDCRLFEDT FVNFQAAIEK KIHASQQRWQ QLKEEIELLQ DLKQTLCSFQ ENRDL
- Concentration
- 0.28 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of SW480 cells, using PHF11 Polyclonal antibody (Product # PA5-89229) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PHF11 in extracts of SW480 cells using PHF11 Polyclonal Antibody (Product # PA5-89229) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PHF11 was performed in A549 cells using PHF11 Polyclonal Antibody (Product # PA5-89229).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PHF11 in paraffin-embedded mouse lung using PHF11 Polyclonal Antibody (Product # PA5-89229) at a dilution of 1:200.
