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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP02205PU-N - Provider product page
- Provider
- OriGene
- Product name
- Relaxin 2 (RLN2) rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- Relaxin 2 (RLN2) rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- RLN2
- Antibody clone number
- NULL
- Vial size
- 100 µg
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ImmunoBlot analysis of Relaxin: Synthetic H1 (100 ng) and Recombinant H2 (500 ng) Relaxin were separated by SDS-PAGE and immunoblotted with AP02205PU relaxin antibody (1/200). Dschietzig T et al. (2001) FASEB Vol 15:2187-95
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ELISA: Standard curve showing measurement of Human Relaxin in a Sandwich Immunoassay using AP02205PU as detection antibody. OD was deteremined at 450 nm. The shown standard curve is for demonstration purposes only and can be influenced by Buffer, incubation conditions or dilution of the conjugate.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry using AP02205PU Ralaxin Antibody: Staining of Paraffin Sections of left ventricular tissue from a patient demonstrating dilated cardiomyopathy. The section was incubated with AP02205PU (1/300) for 24 h. DAB was used as the chromogen. The section was counterstained with Hematoxylin and Eosin. B: AP02205PU stains myocytes (not shown) and interstitial cells (asterisk) of the Myocardium. D: Control section incubated with serum from non-immunized Rabbit. Dschietzig T et al. (2001) FASEB Vol 15:2187-95
- Validation comment
- IHC
