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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- MA5-24088 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Relaxin 2 Monoclonal Antibody (349102)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody detects both the pro and mature forms of human Relaxin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Relaxin-1 or rhRelaxin-3 is observed. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 349102
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human placenta. PVDF membrane was probed with 2 µg/mL of Rat Anti-human Relaxin-2 Monoclonal Antibody (Product # MA5-24088) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. Specific bands were detected for Relaxin-2 at approximately 8 kDa and 27 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Relaxin 2 in human placenta. Samples were incubated in Relaxin 2 monoclonal antibody (Product # MA5-24088) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Rat IgG secondary antibody. Specific bands were detected for Relaxin-2 at approximately 8 kDa and 27 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of Relaxin 2 in THP‚1 human acute monocytic leukemia cell line. Samples were incubated in Relaxin 2 monoclonal antibody (Product # MA5-24088). Recombinant Human Relaxin‚2 induces cAMP production in the THP‚1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line), as measured by cAMP Parameter Assay Kit. cAMP production elicited by Recombinant Human Relaxin‚2 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human Relaxin‚2 Monoclonal Antibody. The ND50 is typically 1.5‚6.0 µg/mL.
