Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 692302 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#692302, RRID:AB_2632763
- Product name
- Purified anti-MAFB
- Antibody type
- Monoclonal
- Description
- MAFB belongs to the Maf transcription factor family, the members of which contain basic leucine zipper (bZIP) domains that bind to DNA. These bZIP domains are located within the Maf recognition element (MARE) and regulate the transcription by binding to acidic domains within the target genes. MAFB plays roles in differentiation processes, including the differentiation of macrophage, pancreatic alpha and beta cells, podocytes in the renal glomerulus, and rhombomeres in the embryonic brain. MAFB is also central to embryonic thymus development, parathyroid gland development, the creation of hair cuticles, and urethral masculinization. In the hematopoietic system, MAFB is important for myeloid lineage commitment of hematopoietic stem cells. MAFB was recently reported to have features of oncogene. It was viewed as a sensitive and specific marker for poor prognosis in multiple myeloma patients. Silencing of MAFB inhibited migration and invasion of nasopharyngeal carcinoma cells. In liver cancer, MAFB promotes cancer cell proliferation by transcriptionally targeting Cyclin D1.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- O91E9
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total protein extracts (15 µg protein) from U87-MG and HepG2 (negative control) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-MAFB (clone O91E9) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
- Conjugate
- Unconjugated