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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-40756 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAFB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: MAAELSMGPE LPTSPLAMEY VNDFDLLKFD VKKEPLGRAE RPGRPCTRLQ Sequence homology: Cow: 100%; Dog: 85%; Guinea Pig: 100%; Horse: 77%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 82%; Zebrafish: 91%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Transcriptional and Histochemical Signatures of Bone Marrow Mononuclear Cell-Mediated Resolution of Synovitis.
Knockout of mafba Causes Inner-Ear Developmental Defects in Zebrafish via the Impairment of Proliferation and Differentiation of Ionocyte Progenitor Cells.
Menarim BC, El-Sheikh Ali H, Loux SC, Scoggin KE, Kalbfleisch TS, MacLeod JN, Dahlgren LA
Frontiers in immunology 2021;12:734322
Frontiers in immunology 2021;12:734322
Knockout of mafba Causes Inner-Ear Developmental Defects in Zebrafish via the Impairment of Proliferation and Differentiation of Ionocyte Progenitor Cells.
Chen X, Huang Y, Gao P, Lv Y, Jia D, Sun K, Han Y, Hu H, Tang Z, Ren X, Liu M
Biomedicines 2021 Nov 16;9(11)
Biomedicines 2021 Nov 16;9(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of transient overexpression lysate of MAFB cells using an anti-MAFB polyclonal antibody (Product # PA5-40756). LANE 1) Transient overexpression lysate of MAFB; LANE 2) Non-overexpressed vector control lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human kidney tissue using an anti-MAFB polyclonal antibody (Product # PA5-40756).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 CRISPR/Cas9-mediated mutagenesis of mafba. ( A ) The sole exon of two mafba transcripts and the CRISPR/Cas9 target site are shown. The 5'UTR, CDS, and 3'UTR regions of the two mafba transcripts are shown in detail. ( B ) DNA sequencing identifies the c.298_304delACTCCTA mafba mutation lines. The 7-bp deletion is indicated with a black frame. ( C ) Relative expressions of mafba determined by qRT-PCR analysis in sibling, mafba +/- , and mafba -/- embryos at 4 dpf. The beta-actin served as the endogenous control. Data are represented as mean +- SD; *, p < 0.05; **, p < 0.01. ( D ) Western blot analysis shows the similar protein expression of Mafba between siblings and mafba +/- embryos, but a significant decrease in mafba -/- mutants at 4 dpf. GAPDH was used as the internal control.
