A700-046-T

antibody from Invitrogen Antibodies

Targeting: MAFB KRML

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-046-T

Provider

Invitrogen Antibodies

Product name

MafB Recombinant Rabbit Monoclonal Antibody (BLR046F)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR046F

Vial size

10 µL

Concentration

1000 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MafB by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, SK-N-MC, RPMI-8226, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafB recombinant monoclonal antibody [BLR046F] (Product # A700-046 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin antibody (Product # A700-057).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse MafB by western blot. Samples: Whole cell lysate (50 µg) from Beta-TC-6 and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafB recombinant monoclonal antibody [BLR046F] (Product # A700-046 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panels: Rabbit anti-MafA recombinant monoclonal antibody [BLR067G] (Product # A700-067) and rabbit anti-Actin recombinant monoclonal antibody [BLR046G] (Product # A700-046).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MAFB in FFPE SK-N-MC cells by immunocytochemistry. Antibody: Rabbit anti-MafB recombinant monoclonal antibody [BLR046F] (Product # A700-046; lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MafB by immunohistochemistry. AntibodySample: FFPE section of human pancreatic tumor.Antibody: Rabbit anti-MafB recombinant monoclonal antibody (BLR046F) (A700-046 lot 1) used at 1:500.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MAFB in FFPE pancreatic tumor by immunohistochemistry. Antibody: Rabbit anti-MafB recombinant monoclonal antibody [BLR046F] (Product # A700-046 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MafB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-MC cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MafB recombinant monoclonal antibody [BLR046F] (Product # A700-046 lot 3) used for IP at 6 µL/mg lysate. MafB was also immunoprecipitated by a previous lot of this antibody (A700-046 lot 2). For blotting immunoprecipitated MafB (Product # A700-046) was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.