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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- MAB3810 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat MafB Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and rat MafB at in Western blots. No cross-reactivity with recombinant human (rh) MafF, rhMafG, or rhMafK is observed.
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y5Q3- Isotype
- IgG
- Antibody clone number
- 372239
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The transcription factor MafB promotes anti-inflammatory M2 polarization and cholesterol efflux in macrophages.
Kim H
Scientific reports 2017 Aug 8;7(1):7591
Scientific reports 2017 Aug 8;7(1):7591
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Rat MafB by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Rat MafB Monoclonal Antibody (Catalog # MAB3810) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MafB, MafF, MafG, and MafK were included. A specific band for MafB was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MafB by Simple WesternTM. Simple Western lane view shows lysates of MDA-MB-468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for MafB at approximately 53 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Rat MafB Monoclonal Antibody (Catalog # MAB3810). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MafB in HepG2 Human Cell Line. MafB was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human/Rat MafB Monoclonal Antibody (Catalog # MAB3810) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
