Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [3]
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Validation data
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- Product number
- NBP2-47793-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TOP1MT Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Analysis of human Jurkat cell lysate using TOP1MT Antibody (TOP1MT/488).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Pancreas stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Gallbladder stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - An intracellular stain was performed on MCF7 cells with TOP1MT Antibody [TOP1MT/488] NBP2-47793AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Analysis of PFA-fixed MCF-7 cells using TOP1MT Antibody (TOP1MT/488).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - An intracellular stain was performed on U2OS cells with TOP1MT Antibody [TOP1MT/488] NBP2-47793AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.