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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21287 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cofilin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, HeLaS3, HepG2, Molt-4, Raji, Neuro2A, C8D30, Raw264.7. Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Cofilin 2 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cofilin 2 Polyclonal Antibody (Product # PA5-21287) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cofilin 2 Polyclonal Antibody (Product # PA5-21287). Sample (30 µg of whole cell lysate). A: Raji. 12% SDS PAGE. Cofilin 2 Polyclonal Antibody (Product # PA5-21287) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cofilin 2 in paraformaldehyde-fixed HeLa cells using a Cofilin 2 polyclonal antibody (Product # PA5-21287) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cofilin 2 antibody (Product # PA5-21287) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Cofilin 2 (Product # PA5-21287) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
