Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-22301 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cofilin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, HCT116, Mouse brain, rat heart.
- Concentration
- 0.28 mg/mL
Submitted references The non-muscle ADF/cofilin-1 controls sarcomeric actin filament integrity and force production in striated muscle laminopathies.
Vignier N, Chatzifrangkeskou M, Pinton L, Wioland H, Marais T, Lemaitre M, Le Dour C, Peccate C, Cardoso D, Schmitt A, Wu W, Biferi MG, Naouar N, Macquart C, Beuvin M, Decostre V, Bonne G, Romet-Lemonne G, Worman HJ, Tedesco FS, Jégou A, Muchir A
Cell reports 2021 Aug 24;36(8):109601
Cell reports 2021 Aug 24;36(8):109601
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cofilin 2 using 30 µg of A) H1299 and B) MOLT4 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Cofilin 2 polyclonal antibody (Product # PA5-22301) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Cofilin 2 was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cofilin 2 Polyclonal Antibody (Product # PA5-22301) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cofilin 2 Polyclonal Antibody (Product # PA5-22301). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. Cofilin 2 Polyclonal Antibody (Product # PA5-22301) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cofilin 2 Polyclonal Antibody (Product # PA5-22301). Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 2 Polyclonal Antibody (Product # PA5-22301) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cofilin 2 in paraformaldehyde-fixed HeLa cells using a Cofilin 2 polyclonal antibody (Product # PA5-22301) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2500.