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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00006925-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006925-M03, RRID:AB_804975
- Product name
- TCF4 monoclonal antibody (M03), clone 1G4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant TCF4.
- Antigen sequence
MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKN
GPTSLASGHFTGSNVEDRSSSGSWGNGGHPSPSRN
YGDGTPYDHTTSRDLGSHDNLSPPFVNSRIQSKTE
RGSYSSYGRESNLQGCHQQSLLGGDMDMGNPGTLS
PTKPGSQYYQYSSNNPRRRPLHSSAMEVQTKKVRK
VPPGLPSSVYAPSASTADYNRDSPGYPSSKPATST
FPSSFFMQDGHHSSDPWSSSSGMNQPGYAGMLGNS
SHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPM
STFHRSGTNHYSTSSCTPPANGTDSIMANRGSGAA
GSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVG
SPPSLSAGTAVWSRN- Isotype
- IgG
- Antibody clone number
- 1G4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Knockdown of human TCF4 affects multiple signaling pathways involved in cell survival, epithelial to mesenchymal transition and neuronal differentiation.
Inhibition of endothelial cell activation by bHLH protein E2-2 and its impairment of angiogenesis.
Forrest MP, Waite AJ, Martin-Rendon E, Blake DJ
PloS one 2013;8(8):e73169
PloS one 2013;8(8):e73169
Inhibition of endothelial cell activation by bHLH protein E2-2 and its impairment of angiogenesis.
Tanaka A, Itoh F, Nishiyama K, Takezawa T, Kurihara H, Itoh S, Kato M
Blood 2010 May 20;115(20):4138-47
Blood 2010 May 20;115(20):4138-47
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TCF4 monoclonal antibody (M03), clone 1G4 Western Blot analysis of TCF4 expression in K-562 ( Cat # L009V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TCF4 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to TCF4 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
