11685-1-AP

antibody from Invitrogen Antibodies

Targeting: PLEK2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11685-1-AP

Provider

Invitrogen Antibodies

Product name

PLEK2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EERDAWAFE ITGAIHAGQP GKVQQLHSLR NSFKLPPHIS LHRIVDKMHD SNTGIRSSPN MEQGSTYKKT FLGSSLVDWL ISNSFTASRL EAVTLASMLM EENFLRPVGV RSMGAIRSGD LAEQFLDDST ALYTFAESYK KKISPKEEIS LSTVELSGTV VKQGYLAKQG HKRKNWKVRR FVLRKDPAFL HYYDPSKEEN RPVGGFSLRG SLVSALEDNG VPTGVKGNVQ GNLFKVITKD DTHYYIQASS KAERAEWIEA IKKLT (90-353 aa encoded by B C001226)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.45 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

U2OS cells were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

U2OS (positive), Jurkat, Ramos, and U-937 (negative) cells were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HT-29 cells were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Caco-2 cells were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using PLEK2 antibody (Product # 11685-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 11685-1-AP (PLEK2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse spleen tissue slide using 11685-1-AP (PLEK2 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse spleen tissue slide using 11685-1-AP (PLEK2 Antibody) at dilution of 1:200 (under 40x lens).