Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30884 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEMA5A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG. Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (83%), Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Semaphorin 5A drives melanoma progression: role of Bcl-2, miR-204 and c-Myb.
D'Aguanno S, Valentini E, Tupone MG, Desideri M, Di Martile M, Spagnuolo M, Buglioni S, Ercolani C, Falcone I, De Dominici M, Milella M, Rizzo MG, Calabretta B, Cota C, Anichini A, Trisciuoglio D, Del Bufalo D
Journal of experimental & clinical cancer research : CR 2018 Nov 19;37(1):278
Journal of experimental & clinical cancer research : CR 2018 Nov 19;37(1):278
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SEMA5A Polyclonal Antibody (Product # PA5-30884). Sample (30 µg of whole cell lysate). Lane A: U87-MG . 5% SDS PAGE. SEMA5A Polyclonal Antibody (Product # PA5-30884) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Semaphorin 5A (Product # PA5-30884) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.