LS-C185416

antibody from LSBio

Targeting: PAX8

Western blot (Enhanced validation)

Immunocytochemistry (Enhanced validation)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C185416

Provider

LSBio

Product name

PAX8 Antibody (aa1-204) LS-C185416

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug whole cell lysate). A:293T. 10% SDS PAGE. PAX8 antibody diluted at 1:1000

Immunocytochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence of paraformaldehyde-fixed A549 using PAX8 antibody at 1:200 dilution.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence of paraformaldehyde-fixed A549 using PAX8 antibody at 1:200 dilution.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence of paraformaldehyde-fixed A549 using PAX8 antibody at 1:200 dilution.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 ug of PAX8 antibody 10% SDS-PAGE The immunoprecipitated PAX8 protein was detected by PAX8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 ug of PAX8 antibody 10% SDS-PAGE The immunoprecipitated PAX8 protein was detected by PAX8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 ug of PAX8 antibody 10% SDS-PAGE The immunoprecipitated PAX8 protein was detected by PAX8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

PAX8 antibody detects PAX8 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. PAX8 antibody dilution:1:500.