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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- TA308698 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal antibody to PAX8CC (paired box 8)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody to PAX8CC (paired box 8)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PAX8
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 1mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample(30 ug whole cell lysate). A:293T. 10% SDS PAGE. TA308698 diluted at 1:1000
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A549, using PAX8(TA308698) antibody at 1:200 dilution.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 ug of PAX8
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- PAX8 antibody detects PAX8 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. PAX8 antibody (TA308698) dilution: 1:500.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of PAX8 antibody (TA308698) The precipitated DNA was detected by PCR with primer set targeting to E2F1
- Validation comment
- Assay
