H00007849-M07

antibody from Abnova Corporation

Targeting: PAX8

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007849-M07

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007849-M07, RRID:AB_581747

Product name

PAX8 monoclonal antibody (M07), clone 1A6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PAX8.

Antigen sequence

MPHNSIRSGHGGLNQLGGAFVNGRPLPEVVRQRIV
DLAHQGVRPCDISRQLRVSHGCVSKILGRYYETGS
IRPGVIGGSKPKVATPKVVEKIGDYKRQNPTMFAW
EIRDRLLAEGVCDNDTVPSVSSINRIIRTKVQQPF
NLPMDSCVATKSLSPGHTLIPSSAVTPPESPQSDS
LGSTYSINGLLGIAQPGSDKRKMDDSDQDSCRLSI
DSQSSSSGPRKHLRTDAFSQHHLEPLECPFERQHY
PEAYASPSHTKGEQGLYPLPLLNSTLDDGKATLTP
SNTPLGRNLSTHQTYPVVADPHSPFAIKQETPEVS
SSSSTPSSLSSSAFLDLQQVGSGVPPFNAFPHAAS
VYGQFTGQALLSGREMVGPTLPGYPPHIPTSGQGS
YASSAIAGMVAGSEYSGNAYGHTPYSSYSEAWRFP
NSSLLSSPYYYSSTSRPSAPPTTATAFDHL


Isotype

IgG

Antibody clone number

1A6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PAX8 expression in transfected 293T cell line by PAX8 monoclonal antibody (M07), clone 1A6.Lane 1: PAX8 transfected lysate(48.119 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PAX8 is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PAX8 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol