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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-7603 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-PAX8 Antibody
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant protei
n (a.a 28-261) of PAX8 was used a
s the immunogen for this antibody.- Isotype
- IgG
- Antibody clone number
- ABM5F33
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of PAX8. Anti-PAX8 antibody (Clone: ABM5F33) was used at 2 µg/ml on Ramos lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flow analysis of Pax8 in Raji cells using 0.5 µg/10^6 cells of antibody (Clone: ABM5F33). Green represents isotype control; red represents anti-Pax8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).
- Protocol
- Protocol
