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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- LS-C761823 - Provider product page
- Provider
- LSBio
- Product name
- PAX8 Antibody (clone PAX8/1492) LS-C761823
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- PAX8/1492
- Storage
- Store at 2-8°C (with azide) or aliquot and store at -20°C (without azide).
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot testing of 1) human partial recombinant protein and 2) human Raji cell lysate with PAX8 antibody (clone PAX8/1492). Predicted molecular weight of isoforms 1-5: 31, 35, 42, 43 and 48 kDa, respectively. PAX8 can also be observed at ~62 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC testing of FFPE human urothelial carcinoma with PAX8 antibody (clone PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC testing of FFPE human renal cell carcinoma with PAX8 antibody (clone PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC testing of FFPE human thyroid carcinoma with PAX8 antibody (clone PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC testing of FFPE human thyroid carcinoma with PAX8 antibody (clone PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC testing of FFPE human urothelial carcinoma with PAX8 antibody (clone PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC testing of FFPE human renal cell carcinoma with PAX8 antibody (clone PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.
