PA5-21368

antibody from Invitrogen Antibodies

Targeting: PAX8

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21368

Provider

Invitrogen Antibodies

Product name

PAX8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, 293T nuclear extract, DDDDK-tagged PAX8-transfected 293T. Predicted reactivity: Mouse (98%), Rat (99%), Xenopus laevis (91%), Dog (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.87 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PAX8C in paraformaldehyde-fixed A549 cells using a PAX8C polyclonal antibody (Product # PA5-21368) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PAX8 (Product # PA5-21368) antibody at 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PAX8 was performed using 70% confluent log phase SK-OV-3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with PAX8 Polyclonal Antibody (Product # PA5-21368) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents IMR-32 cells having no expression of PAX8. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PAX8 Polyclonal Antibody detects PAX8 protein at cytosol and nucleus on rat thyroid gland by immunohistochemical analysis. Sample: Paraffin-embedded rat thyroid gland. PAX8 Polyclonal Antibody (Product # PA5-21368) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of PAX8 was performed in paraffin-embedded DLD1 xenograft tissue using PAX8 Polyclonal Antibody (Product # PA5-21368) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of PAX8 was performed in paraffin-embedded mouse liver tissue using PAX8 Polyclonal Antibody (Product # PA5-21368) at a dilution of 1:500.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract. A: 5 µg preimmune rabbit IgG. B: 5 µg of PAX8 antibody (Product # PA5-21368). The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PAX8 Polyclonal Antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample: 1,000 µg 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 µg of PAX8 Polyclonal Antibody (Product # PA5-21368) 10% SDS-PAGE The immunoprecipitated PAX8 protein was detected by PAX8 Polyclonal Antibody (Product # PA5-21368) diluted at 1:1,000.