NBP1-32440
antibody from Novus Biologicals
Targeting: PAX8
Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NBP1-32440 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32440, RRID:AB_2283498
- Product name
- Rabbit Polyclonal PAX8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references HBx regulates transcription factor PAX8 stabilization to promote the progression of hepatocellular carcinoma.
Renal cancer: new models and approach for personalizing therapy.
BCOR-CCNB3 Undifferentiated Sarcoma-Does Immunohistochemistry Help in the Identification?
The PAX8 cistrome in epithelial ovarian cancer.
GLIS3 is indispensable for TSH/TSHR-dependent thyroid hormone biosynthesis and follicular cell proliferation.
Epigenetic remodeling regulates transcriptional changes between ovarian cancer and benign precursors.
Renal progenitors derived from human iPSCs engraft and restore function in a mouse model of acute kidney injury.
Wang J, Li N, Huang ZB, Fu S, Yu SM, Fu YM, Zhou PC, Chen RC, Zhou RR, Huang Y, Hu XW, Fan XG
Oncogene 2019 Oct;38(40):6696-6710
Oncogene 2019 Oct;38(40):6696-6710
Renal cancer: new models and approach for personalizing therapy.
di Martino S, De Luca G, Grassi L, Federici G, Alfonsi R, Signore M, Addario A, De Salvo L, Francescangeli F, Sanchez M, Tirelli V, Muto G, Sperduti I, Sentinelli S, Costantini M, Pasquini L, Milella M, Haoui M, Simone G, Gallucci M, De Maria R, Bonci D
Journal of experimental & clinical cancer research : CR 2018 Sep 5;37(1):217
Journal of experimental & clinical cancer research : CR 2018 Sep 5;37(1):217
BCOR-CCNB3 Undifferentiated Sarcoma-Does Immunohistochemistry Help in the Identification?
Ludwig K, Alaggio R, Zin A, Peron M, Guzzardo V, Benini S, Righi A, Gambarotti M
Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society 2017 Jul-Aug;20(4):321-329
Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society 2017 Jul-Aug;20(4):321-329
The PAX8 cistrome in epithelial ovarian cancer.
Adler EK, Corona RI, Lee JM, Rodriguez-Malave N, Mhawech-Fauceglia P, Sowter H, Hazelett DJ, Lawrenson K, Gayther SA
Oncotarget 2017 Dec 12;8(65):108316-108332
Oncotarget 2017 Dec 12;8(65):108316-108332
GLIS3 is indispensable for TSH/TSHR-dependent thyroid hormone biosynthesis and follicular cell proliferation.
Kang HS, Kumar D, Liao G, Lichti-Kaiser K, Gerrish K, Liao XH, Refetoff S, Jothi R, Jetten AM
The Journal of clinical investigation 2017 Dec 1;127(12):4326-4337
The Journal of clinical investigation 2017 Dec 1;127(12):4326-4337
Epigenetic remodeling regulates transcriptional changes between ovarian cancer and benign precursors.
Elias KM, Emori MM, Westerling T, Long H, Budina-Kolomets A, Li F, MacDuffie E, Davis MR, Holman A, Lawney B, Freedman ML, Quackenbush J, Brown M, Drapkin R
JCI insight 2016 Aug 18;1(13)
JCI insight 2016 Aug 18;1(13)
Renal progenitors derived from human iPSCs engraft and restore function in a mouse model of acute kidney injury.
Imberti B, Tomasoni S, Ciampi O, Pezzotta A, Derosas M, Xinaris C, Rizzo P, Papadimou E, Novelli R, Benigni A, Remuzzi G, Morigi M
Scientific reports 2015 Mar 6;5:8826
Scientific reports 2015 Mar 6;5:8826
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PAX8 Antibody [NBP1-32440] - Simple Western lane view shows a specific band for PAX8 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PAX8 Antibody [NBP1-32440] - 293T whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PAX8 Antibody [NBP1-32440] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of PAX8 protein by 2 ug of PAX8 antibody 10% SDS-PAGE gel.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Paraffin-embedded DLD1 xenograft . PAX8 antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Paraffin-embedded mouse liver. PAX8 antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Paraffin-embedded rat thyroid gland dilution: 1:500.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: PAX8 Antibody [NBP1-32440] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of PAX8 antibody The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.