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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
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- Product number
- NB100-1065 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1065, RRID:AB_2159974
- Product name
- Goat Polyclonal PAX8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Pax8 has a key role in thyroid cell differentiation.
Pasca di Magliano M, Di Lauro R, Zannini M
Proceedings of the National Academy of Sciences of the United States of America 2000 Nov 21;97(24):13144-9
Proceedings of the National Academy of Sciences of the United States of America 2000 Nov 21;97(24):13144-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PAX8 Antibody [NB100-1065] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PAX8 Antibody [NB100-1065] - Staining of Human Kidney (A) and HeLa cell (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PAX8 Antibody [NB100-1065] - IHC analysis of PAX8 in paraffin embedded Human Thyroid Gland using this antibody at 3ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PAX8 Antibody [NB100-1065] - Human Human Thyroid Gland.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PAX8 Antibody [NB100-1065] - (4ug/ml) staining of paraffin embedded Human Thyroid Gland. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PAX8 Antibody [NB100-1065] - Staining of paraffin embedded Human Kidney Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
