AF8035

antibody from R&D Systems

Targeting: ITGB5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF8035

Provider

R&D Systems

Product name

Mouse/Rat Integrin beta 5 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse and rat in Western blots.

Reactivity

Mouse, Rat

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

NP_001139356


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse and Rat Integrin beta 5 by Western Blot. Western blot shows lysates of CMT-93 mouse rectal carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8035) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin beta 5 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Integrin beta 5 in Mouse Brain. Integrin beta 5 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse/Rat Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8035) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010). Specific staining was localized to glial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Integrin beta 5 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Sheep Anti-Mouse/Rat Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8035, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).