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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- PA5-49745 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ITGB5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total Integrin beta 5 protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ITGB5 in extracts from COS7 cells. Samples were probed using ITGB5 antibody (Product # PA5-49745).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ITGB5 in extracts from JK cells (Lane 2) and cos-7 cells (Lane 3). Samples were probed using ITGB5 antiobdy (Product # PA5-49745).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of ITGB5 was achieved by transfecting A549 with ITGB5 specific siRNA (Silencer® select Product # s7590). Western blot analysis (Fig. a) was performed using membrane enriched extracts from ITGB5 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with ITGB5 Polyclonal Antibody (Product # PA5-49745, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ITGB5.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ITGB5 Polyclonal Antibody (Product # PA5-49745) and a 88 kDa band corresponding to ITGB5 was observed across cell lines tested and increased upon TGF beta treatment, along with an uncharacterized band (*) ~ 70 kDa in tissues tested. Membrane enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with TGF beta (2 ng/mL for 24 Hours) (Lane 2), A549 (Lane 3) and tissue extracts (30 ug lysate) of Mouse Kidney (Lane 4) and Mouse Brain (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
